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Like most proteins, they are synthesized by the ribosomes in the cell. They react with a specific substrate in order to increase the rate of a chemical reaction within the cell. Without enzymes, reactions would be significantly slower and we would not be able to do the most basic functions, such as breathing or digesting food.
Amylase is an type of enzyme. It breaks down starch to Chardakovs banana experiment essay, giving food that sweet taste.
An example of amylase in the natural world is in bananas. This is why brown bananas taste sweeter than their green counterpart. Within this experiment, the objective was to test how temperature, pH level and enzyme concentration changed the effectiveness of amylase.
Methods First, an indicator experiment was performed. In two test tubes, a 1 mL millimeter sample of starch solution was pipetted.
The same was done with 1 mL of maltose solution into another two tubes. Into one of the test tubes of maltose and one of starch, 5 drops of I2KI were added. All four tubes were then watched for color change, indicating a reaction. The activity of amylase was then observed through three reaction mixtures.
Immediately a drop of each mixture was transferred to a separate well on a spot plate and a drop of I2KI was added. This was then repeated every minute. The color was observed with the passage of time to conclude whether amylase activity was present or not. The tubes were observed for color change, indicating the presence of maltose.
Then, 1 mL of the temperature equilibrated starch was added. Immediately a drop of the reaction was transferred to the spot plate and 1 drop of I2KI was added. This step was repeated every minute to test the presence of starch. Complete this process for all three temperatures. Next, pH levels were tested.
Four test tubes were filled with 1 mL of pH 4, 5, 6 and 7 starch solutions respectively. This was repeated at one minute intervals. Finally, the effects of enzyme concentration were tested.
One mL of pH 7 starch solution was pipetted into three test tubes. One drop of each mixture was immediately transferred to the spot plate and a drop of I2KI was added. This was repeated every minute. Results Within the indicators experiment, the first test tube changed from clear to dark purple.
The second tube changed from blue to yellow. Tube 2 indicated starch for all 8 minutes. Tube 3 never indicated starch. Temperature seemed to have a positive correlations with the speed of the reaction.
This is shown in Figure 1. Maltose was shown to be present in all three test tubes. Test tubes 2 and 3, which contained pH 5 and 6 starch, completed the reaction at the same time.
Figure 2 shows the reaction rates of the different pH levels. Maltose was present in all test tubes. Figure 3 shows the direct correlation. All three tubes had maltose present.
The Effects of Enzyme Concentration on Activity Discussion In the indicator experiment, it was necessary to test what each indicator marked. As expected, as the temperature increased, so did the speed of the reaction.
The results of the pH experiment did not clearly show what the best level for the enzyme was.3. two plastic bags Abstract Experiment Results In one week, the bread mold is just hard, but the cheese mold has spread covering almost half. In 2 weeks the bread hasn't changed much but the cheese is halfway covered in mold.
In 3 weeks the bread is half .
September 23, Purpose: The purpose of the experiment was to perform the acid-catalyzed Fischer Esterification of acetic acid and isopentyl alcohol to form isopentyl acetate, or banana oil, which is used in flavor industries.
The equilibrium of the reaction was changed by adding an excess amount of acetic acid. 1. Abstract of experiment. (10 points) This is a summary of the basic content of the experiment.
It should state the purpose of the experiment, mention the techniques used, report results obtained, and give conclusions. The point of the abstract is to give a concise summary of the whole report. In this experiment, how do the Lysis Solution and the Salt Solution vary by function Identify one step which was included in the Virtual Lab which was not required in the hands-on experiment.
Then, identify one step which was included in the hands-on experiment, but not the virtual lab. The results of the pH experiment did not clearly show what the best level for the enzyme was.
The concentration experiment was much more clear. The 20% concentration reacted the fastest because there was more enzymes to react with the substrate and create maltose. Literature Cited.
This simple experiment will show you how to extract DNA from fruit like a banana or strawberry. June 4, Deoxyribonucleic Acid Extraction from Commercial Strawberries Priyank Patel Course Code: SBI4U—Period 2 Student Number.